ISBN:
9781461538202
Language:
English
Pages:
Online-Ressource (xii, 169 p)
,
ill
Edition:
Springer eBook Collection. Humanities, Social Sciences and Law
DDC:
50
Keywords:
Science (General)
Description / Table of Contents:
1 Aspartate Transcarbamylase2 Molecular Genetics: Regulation of Aspartate Transcarbamylase Biosynthesis -- 3 Purification of Aspartate Transcarbamylase and Its Subunits -- 4 Structural and Physicochemical Study of Aspartate Transcarbamylase -- 5 Enzymatic Catalysis and Regulation -- 6 Complementary Experiments -- 1. Equations and Units -- 2. Molar Mass and Molecular Mass -- 3. Units of Catalytic Activity -- 4. Units of Radioactivity -- 5. Units of Quantity -- 7. Calculation of Acceleration -- 8. Bacterial Strains -- 9. Solutions and Reagents -- 9.1. 0.8-M Tris-Acetate Buffer, pH 8 -- 9.2. 0.2-M Acetic Acid -- 9.6. Elution Buffer for Chromatography: 10 × Stock Solution -- 9.7. 100-mM Aspartate, pH 8 -- 9.8. 10-mM Carbamylaspartate, pH 8 -- 9.9. 1-M Phosphate Buffer, pH 7.2 -- 9.10. Buffer for Dilution of E -- 9.11. Buffer for Dilution of C -- 9.12. Buffer for Dilution of R and Recombination -- 9.13. 200-mM Cacodylate Buffer, pH 6, 7, and 7.5 -- 9.14. 200-mM Tris-Acetate Buffer, pH 8 and 9 -- 9.15. 200-mM Glycine Buffer, pH 10 -- 9.16. 400-mM Succinate, pH 7 -- 9.17. Reaction Medium: 20-mM CAP-200-mM Tris-Acetate, pH 8 -- 10. Preparation of Standard Protein Mixtures for Column Calibration -- Answers To Questions -- References.
DOI:
10.1007/978-1-4615-3820-2
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