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  • ebrary, Inc
  • Dordrecht : Springer
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  • 1
    ISBN: 9789401105118
    Language: English
    Pages: Online-Ressource (1 v. (various pagings)) , ill
    Edition: Second edition
    Edition: Springer eBook Collection. Humanities, Social Sciences and Law
    DDC: 50
    Keywords: Science (General)
    Description / Table of Contents: Section A : In vitro methods of gene transfer to plant cells1. PEG-mediated direct gene transfer and electroporation -- 2. Gene transfer to plants via particle bombardment -- Section B: Agrobacterium-mediated gene transfer to plant cells -- 1. Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems -- 2. Specialized vectors for gene tagging and expression studies -- 3. Agrobacterium molecular genetics -- 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species -- 5. Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer -- 6. Agrobacterium inoculation techniques for plant tissues -- Section C: Selectable and screenable markers for plant transformation -- 1. Antibiotic-resistance markers for plant transformation -- 2. Reporter genes for plants -- 3. Opines as screenable markers for plant transformation -- Section D: Nucleic acid extraction from plant tissue -- 1. Extraction of total cellular DNA from plants, algae and fungi -- 2. Isolation and characterization of nuclear scaffolds -- 3. Isolation of plant mitochondria and mitochondrial nucleic acids -- 4. Isolation of chloroplasts and chloroplast DNA -- 5. Isolation of total, poly (A) and polysomal RNA from plant tissues -- Section E: Transcription and translation systems -- 1. Assay for gene expression using run-on transcription in isolated nuclei -- 2. Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity -- Section F: Blotting and gene detection systems -- 1. Southern, Northern and Western blot analysis -- 2. Screening of cDNA expression libraries with synthetic oligonucleotides for DNA-binding proteins -- 3. Non-radioactive nucleic acid detection systems -- Section G: In situ hybridization and immunodetection -- 1. RNA in situ hybridization in plants -- 2. In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences -- Section H: Cloning and detection of DNA sequences from large DNA molecules -- 1. Methods for generating plant genomic libraries Marjory -- 2. Construction of plant yeast artificial chromosome libraries -- 3. Preparation of high molecular weight plant DNA and analysis by pulsed field gel electrophoresis -- 4. Random amplified polymorphic DNA (RAPD) markers -- Section I: Protein-nucleic acid interaction analyses -- 1. Gel mobility shift assay -- 2. Optimization of DNase I footprinting experiments -- 3. Analyses of plant chromatin and in vivo protein-DNA interactions -- 4. Expression and characterization of recombinant plant trans-acting factors -- Section J: Subcellular targeting of proteins -- 1. In vitro import of proteins into chloroplasts -- 2. In vitro targeting of proteins to mitochondria -- 3. Targeting of proteins to the vacuole -- 4. Visualizing protein import into the plant cell nucleus -- Section K: Gene tagging using transposons -- 1. Gene tagging by endogenous transposons -- 2. Heterologous transposon tagging as a tool for the isolation of plant genes.
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  • 2
    ISBN: 9789401127738
    Language: English
    Pages: Online-Ressource (x, 345 p) , ill
    Edition: Springer eBook Collection. Humanities, Social Sciences and Law
    Series Statement: The GeoJournal Library 21
    DDC: 551.46
    Keywords: Geography ; Hydraulic engineering ; Meteorology ; Oceanography ; Ecology ; Environmental toxicology
    Abstract: This volume presents a collection of original, multidisciplinary papers written by leading scientists from the various countries of the Pacific rim. The major topics covered are the origins of life in the marine environment; the changing distribution of biological parameters; hydrophysical and hydrochemical processes; and the exploitation of ocean resources and the ensuing consequences. The material is divided into five parts. The Introduction (Part I) gives a comprehensive overview of the research activities of the IOC and related organisations. The following four parts of the book comprise a number of survey papers which deal with specific aspects of marine science in the Pacific Ocean. For researchers and decision makers interested in marine and environmental science with an emphasis on the Pacific Ocean
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  • 3
    ISBN: 9789401135061
    Language: English
    Pages: Online-Ressource (xii, 349 p) , ill
    Edition: Springer eBook Collection. Humanities, Social Sciences and Law
    Parallel Title: Erscheint auch als
    Parallel Title: Erscheint auch als
    Keywords: Humanities ; Computer science ; Social sciences ; User interfaces (Computer systems). ; Human-computer interaction.
    Abstract: Riding a Tiger, or Computer Supported Cooperative Work -- Personalisable Groupware: Accommodating Individual Roles and Group Differences -- Office Systems Development and Gender: Implications for Computer-Supported Co-operative Work -- CSCW and Distributed Systems: The Problem of Control -- Collaborative Activity and Technological Design: Task Coordination in London Underground Control Rooms -- The Group Facilitator: A CSCW Perspective -- Idea Management in a Shared Drawing Tool -- Panel: Formalization in CSCW -- Experiences with the DOMINO Office Procedure System -- Distributed Computing and Organisational Change Enable Concurrent Engineering -- An Analysis of Design and Collaboration in a Distributed Environment -- ClearFace: Translucent Multiuser Interface for TeamWorkStation -- PEPYS: Generating Autobiographies by Automatic Tracking -- Panel: Organizational Memory -- Boosting Connectivity in a Student Generated Collaborative Database -- A Model for Real-Time Co-operation -- Questioning Representations -- Speech Acts or Communicative Action? -- The Concept of Activity as a Basic Unit of Analysis for CSCW Research -- Being Selectively Aware with the Khronika System -- Participation Frameworks for Computer Mediated Communication -- Sound Support for Collaboration -- CSCW: Discipline or Paradigm? A Sociological Perspective -- Small Workshop Abstracts -- ECSCW’91 Directory: Authors & Committee Members.
    Description / Table of Contents: Riding a Tiger, or Computer Supported Cooperative WorkPersonalisable Groupware: Accommodating Individual Roles and Group Differences -- Office Systems Development and Gender: Implications for Computer-Supported Co-operative Work -- CSCW and Distributed Systems: The Problem of Control -- Collaborative Activity and Technological Design: Task Coordination in London Underground Control Rooms -- The Group Facilitator: A CSCW Perspective -- Idea Management in a Shared Drawing Tool -- Panel: Formalization in CSCW -- Experiences with the DOMINO Office Procedure System -- Distributed Computing and Organisational Change Enable Concurrent Engineering -- An Analysis of Design and Collaboration in a Distributed Environment -- ClearFace: Translucent Multiuser Interface for TeamWorkStation -- PEPYS: Generating Autobiographies by Automatic Tracking -- Panel: Organizational Memory -- Boosting Connectivity in a Student Generated Collaborative Database -- A Model for Real-Time Co-operation -- Questioning Representations -- Speech Acts or Communicative Action? -- The Concept of Activity as a Basic Unit of Analysis for CSCW Research -- Being Selectively Aware with the Khronika System -- Participation Frameworks for Computer Mediated Communication -- Sound Support for Collaboration -- CSCW: Discipline or Paradigm? A Sociological Perspective -- Small Workshop Abstracts -- ECSCW’91 Directory: Authors & Committee Members.
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