ISBN:
9789401105118
Language:
English
Pages:
Online-Ressource (1 v. (various pagings))
,
ill
Edition:
Second edition
Edition:
Springer eBook Collection. Humanities, Social Sciences and Law
DDC:
50
Keywords:
Science (General)
Description / Table of Contents:
Section A : In vitro methods of gene transfer to plant cells1. PEG-mediated direct gene transfer and electroporation -- 2. Gene transfer to plants via particle bombardment -- Section B: Agrobacterium-mediated gene transfer to plant cells -- 1. Agrobacterium-mediated gene transfer to plant cells: cointegrate and binary vector systems -- 2. Specialized vectors for gene tagging and expression studies -- 3. Agrobacterium molecular genetics -- 4. Genetic manipulation of Agrobacterium tumefaciens strains to improve transformation of recalcitrant plant species -- 5. Transient expression assays using GUS constructs and fluorometric detection for analysis of T-DNA transfer -- 6. Agrobacterium inoculation techniques for plant tissues -- Section C: Selectable and screenable markers for plant transformation -- 1. Antibiotic-resistance markers for plant transformation -- 2. Reporter genes for plants -- 3. Opines as screenable markers for plant transformation -- Section D: Nucleic acid extraction from plant tissue -- 1. Extraction of total cellular DNA from plants, algae and fungi -- 2. Isolation and characterization of nuclear scaffolds -- 3. Isolation of plant mitochondria and mitochondrial nucleic acids -- 4. Isolation of chloroplasts and chloroplast DNA -- 5. Isolation of total, poly (A) and polysomal RNA from plant tissues -- Section E: Transcription and translation systems -- 1. Assay for gene expression using run-on transcription in isolated nuclei -- 2. Preparation of an in vitro transcription system of plant origin, with methods and templates for assessing its fidelity -- Section F: Blotting and gene detection systems -- 1. Southern, Northern and Western blot analysis -- 2. Screening of cDNA expression libraries with synthetic oligonucleotides for DNA-binding proteins -- 3. Non-radioactive nucleic acid detection systems -- Section G: In situ hybridization and immunodetection -- 1. RNA in situ hybridization in plants -- 2. In situ hybridization to plant metaphase chromosomes using digoxigenin labeled nucleic acid sequences -- Section H: Cloning and detection of DNA sequences from large DNA molecules -- 1. Methods for generating plant genomic libraries Marjory -- 2. Construction of plant yeast artificial chromosome libraries -- 3. Preparation of high molecular weight plant DNA and analysis by pulsed field gel electrophoresis -- 4. Random amplified polymorphic DNA (RAPD) markers -- Section I: Protein-nucleic acid interaction analyses -- 1. Gel mobility shift assay -- 2. Optimization of DNase I footprinting experiments -- 3. Analyses of plant chromatin and in vivo protein-DNA interactions -- 4. Expression and characterization of recombinant plant trans-acting factors -- Section J: Subcellular targeting of proteins -- 1. In vitro import of proteins into chloroplasts -- 2. In vitro targeting of proteins to mitochondria -- 3. Targeting of proteins to the vacuole -- 4. Visualizing protein import into the plant cell nucleus -- Section K: Gene tagging using transposons -- 1. Gene tagging by endogenous transposons -- 2. Heterologous transposon tagging as a tool for the isolation of plant genes.
DOI:
10.1007/978-94-011-0511-8
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