ISSN:
0378-8741
Language:
English
Titel der Quelle:
Journal of ethnopharmacology : an interdisciplinary journal devoted to bioscientific research on indigenous drugs
Publ. der Quelle:
Shannon : Elsevier Science Ireland
Angaben zur Quelle:
Vol. 186 (2016), p. 125-135
DDC:
610
Abstract:
Existing evidences suggest that Radix Astragali and its polysaccharides composition (APS) can improve muscle mass, but the mechanisms need more research. In this study, we aimed to examine the effects of APS on muscle wasting at molecular level in 5/6 nephrectomised rats. We performed 5/6 nephrectomy or sham operation in 160 6-week-old Sprague-Dawley rats, and feed animals with or without 2% APS for 155 days. After treatment, we compared the change of weight, muscle fibre, protein metabolism, pro-inflammatory factors (TNF-α, IL-15, CRP) and oxidative factors (MDA, SOD) among each group. In addition, we detected the Akt/mTOR, ubiquitin proteasome, autophagy signalling and AA transporters in vivo and in vitro. Data in vivo show 2% APS could alleviate weight loss and improve protein metabolism in nephrectomised rats. The levels of serum pro-inflammatory factors and oxidative factors were restored by APS treatment. In molecular levels, APS restored Akt/mTOR, MAFbx, MuRF1, Atg7, LC3B-II/LC3B-I and SLC38A2 which changed in nephrectomised rats. Data in vitro show the optimal dose of APS is 0.2mg/mL, and SLC38A2 siRNA attenuated the effects of 0.2mg/mL APS on atrophy and autophagy. Our results suggested APS could improve muscle wasting through Akt/mTOR, ubiquitin proteasome and autophagy signalling, and SLC38A2 may be one of potential targets.
Note:
Copyright: © Elsevier Ireland Ltd
,
Copyright: Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.
DOI:
10.1016/j.jep.2016.03.068
URL:
http://www.sciencedirect.com/science/article/pii/S037887411630191X
URL:
http://www.ncbi.nlm.nih.gov/pubmed/27049295
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